Irx1^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Irx1^{tm1(KOMP)Vlcg}
• Name: Iroquois homeobox 1; targeted mutation 1, Velocigene
• MGI ID: MGI:4399218
• Synonyms: Irx1^-, Irx1^LacZNeo
• Gene: Irx1 Location: Chr13:72106351-72111842 bp, - strand Genetic Position: Chr13, 38.43 cM
• Alliance: Irx1^{tm1(KOMP)Vlcg} page
• IMPC: Irx1 gene page

Mutation origin

• Mutant Cell Lines: 11187B-D3, 11187B-D12, 11187B-E2, 11187B-F4
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 4980bp between positions 72106625-72111604 of Chromosome 13 (Build GRCm39). All 4 exons (i.e. the entire sequence from start codon to stop codon) were replaced with a lacZ reporter and a floxed neomycin resistance cassette. qRT-PCR and Western blot analysis confirmed complete loss of mRNA and protein expression in lung tissue from P0 homozygotes. (J:136110, J:248919)

Expression

In Mice Carrying this Mutation:	53 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Irx1 Mutation: 22 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)