Ldah^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Ldah^{tm1(KOMP)Vlcg}
• Name: lipid droplet associated hydrolase; targeted mutation 1, Velocigene
• MGI ID: MGI:4399711
• Gene: Ldah Location: Chr12:8258107-8335759 bp, + strand Genetic Position: Chr12, 3.85 cM
• Alliance: Ldah^{tm1(KOMP)Vlcg} page
• IMPC: Ldah gene page

Mutation origin

• Mutant Cell Lines: 14003A-A7, 14003A-D9, 14003A-H3
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1 Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 18006bp between positions 8270605-8288610 of Chromosome 12 (Build GRCm39). Sequencing confirmed replacement of a region from exon 2, downstream of the start codon, to base 169 of exon 3, including the predicted catalytic Gx-SxG motif, with a lacZ reporter and a loxP-flanked neomycin cassette. Western blot analysis using an antibody binding a C-terminal antigen revealed no detectable protein in the liver, BAT and WAT, adrenal gland, prostate, brain, spleen, kidney, testis, and bone marrow-derived macrophages of homozygous mutant mice. Mass spectrometry analysis confirmed the absence of encoded peptides in WAT and liver lysates. (J:136110, J:237921)

Generation of the Ldah^tm1(KOMP)Vlcg allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ldah Mutation: 23 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)