Tg(CAG-CYP27A1)23Etl
Transgene Detail

Summary

• Symbol: Tg(CAG-CYP27A1)23Etl
• Name: transgene insertion 23, Eran Leitersdorf
• MGI ID: MGI:4410298
• Synonyms: CYP27^overexp
• Transgene: Tg(CAG-CYP27A1)23Etl Location: unknown
• Alliance: Tg(CAG-CYP27A1)23Etl page

Transgene origin

• Strain of Origin: CBA

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-CYP27A1)23Etl expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CYP27A1 (1593)

  Cyp27a1

  (MGI:88594)

• Mutation details: The transgene construct has the human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector) driving expression of a 1.8 kb fragment encoding the human CYP27A1 (cytochrome P450, family 27, subfamily A, polypeptide 1) cDNA sequence. This transgene was microinjected into the pronuclei of fertilized mouse eggs taken from superovulated CBA females. The highest transgenic overexpressor founder mouse (23) was bred to wildtype C57BL/6J (Harlan) mice to establish line 23. (J:78951)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:78951 Meir K, et al., Human sterol 27-hydroxylase (CYP27) overexpressor transgenic mouse model. Evidence against 27-hydroxycholesterol as a critical regulator of cholesterol homeostasis. J Biol Chem. 2002 Sep 13;277(37):34036-41
• All: 7 reference(s)