Tg(CMV-RNAi:Pqbp1)1Hiok
Transgene Detail

Summary

• Symbol: Tg(CMV-RNAi:Pqbp1)1Hiok
• Name: transgene insertion 1, Hitoshi Okazawa
• MGI ID: MGI:4410523
• Synonyms: PQBP1-KD
• Transgene: Tg(CMV-RNAi:Pqbp1)1Hiok Location: unknown
• Alliance: Tg(CMV-RNAi:Pqbp1)1Hiok page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Knockdown)
• Mutation: Insertion
• Tg(CMV-RNAi:Pqbp1)1Hiok involves 1 genes/genome features (Pqbp1) View all
• Mutation details: A CMV promoter drives ubiquitous expression of a double-stranded RNA construct consisting of a 498 bp anti-sense and sense fragment of Pqbp1 arranged tail to tail preceded by a ribozyme cassette and followed by a MAZ site for RNA Pol II pausing. Two lines were created (lines 1 and 2). Western blot analysis confirmed that both lines exhibit a 50% reduction in endogenous PQBP1 expression without affecting expression of GAPDH, alpha-Tubulin, beta-Actin, GFAP, or MTAP2. Line 1 was selected as the representative line. (J:154081)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:154081 Ito H, et al., Knock-down of PQBP1 impairs anxiety-related cognition in mouse. Hum Mol Genet. 2009 Nov 15;18(22):4239-54
• All: 1 reference(s)