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Del(15Krt7-Krt18)1Tmm
Targeted Allele Detail
Summary
Symbol: Del(15Krt7-Krt18)1Tmm
Name: deletion, Chr 15, Thomas M Magin 1
MGI ID: MGI:4410584
Synonyms: Del(15Krt80-Krt18)1Tmm, KtyII-
Gene: Del(15Krt7-Krt18)1Tmm  Location: Chr15:101246196-101940461 bp  Genetic Position: Chr15, Syntenic
Del(15Krt7-Krt18)1Tmm/Del(15Krt7-Krt18)1Tmm embryos exhibit severe growth retardation and apoptosis

Show the 2 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:153804
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt1b-m2
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutations:    Insertion, Intergenic deletion, Intragenic deletion
  Del(15Krt7-Krt18)1Tmm involves 34 genes/genome features (Mir1941, Gm671, Krt7 ...) View all
 
Mutation detailsLoxP sites were inserted 5' and 3' of the keratin type II gene cluster, encompassing 0.68 Mb, 26 type II genes and 1 keratin type I gene (Krt18). Cre mediated recombination deleted the entire cluster leaving a single loxP site flanked by 3' and 5' hprt mini-genes and the the tyrosinase (Tyr) and agouti (A) coat color genes. The absence of the transcripts and gene products was confirmed by RT-PCR and immunofluorescence microscopy in homozygous embryos. Due to the absence of all type II keratins, all the type I keratins were sensitive to proteolysis, so homozygous mice lack all 54 keratin proteins. ES cells carrying Krt1ctm1Tmm and Igs11tm1Tmm and were used to create this deletion. (J:153804)
Generation of the Del(15Krt7-Krt18)1Tmm deletion
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Del(15Krt7-Krt18)1Tmm Mutation:  0 strains or lines available
References
Original:  J:153804 Vijayaraj P, et al., Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor. J Cell Biol. 2009 Oct 19;187(2):175-84
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory