Del(15Krt7-Krt18)1Tmm
Targeted Allele Detail

Summary

• Symbol: Del(15Krt7-Krt18)1Tmm
• Name: deletion, Chr 15, Thomas M Magin 1
• MGI ID: MGI:4410584
• Synonyms: Del(15Krt80-Krt18)1Tmm, KtyII-
• Gene: Del(15Krt7-Krt18)1Tmm Location: Chr15:101246196-101940461 bp Genetic Position: Chr15, Syntenic

Del(15Krt7-Krt18)1Tmm/Del(15Krt7-Krt18)1Tmm embryos exhibit severe growth retardation and apoptosis

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153804
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutations: Insertion, Intergenic deletion, Intragenic deletion Del(15Krt7-Krt18)1Tmm involves 34 genes/genome features (Mir1941, Gm671, Krt7 ...) View all Mutation details: LoxP sites were inserted 5' and 3' of the keratin type II gene cluster, encompassing 0.68 Mb, 26 type II genes and 1 keratin type I gene (Krt18). Cre mediated recombination deleted the entire cluster leaving a single loxP site flanked by 3' and 5' hprt mini-genes and the the tyrosinase (Tyr) and agouti (A) coat color genes. The absence of the transcripts and gene products was confirmed by RT-PCR and immunofluorescence microscopy in homozygous embryos. Due to the absence of all type II keratins, all the type I keratins were sensitive to proteolysis, so homozygous mice lack all 54 keratin proteins. ES cells carrying Krt1c^tm1Tmm and Igs11^tm1Tmm and were used to create this deletion. (J:153804)

Generation of the Del(15Krt7-Krt18)1Tmm deletion

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(15Krt7-Krt18)1Tmm Mutation: 0 strains or lines available

References

• Original: J:153804 Vijayaraj P, et al., Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor. J Cell Biol. 2009 Oct 19;187(2):175-84
• All: 7 reference(s)