Pparg^tm1.1Unc
Targeted Allele Detail

Summary

• Symbol: Pparg^tm1.1Unc
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 1.1, University of North Carolina
• MGI ID: MGI:4412008
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^tm1.1Unc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154767
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 345 bp fragment containing the 3' UTR was replaced with an 855bp fragment containing the rabbit beta-globin 3' UTR and a self-excising neo cassette via homologous recombination. The self-excising neo resistance cassette was then removed during germline transmission. Quantitative RT-PCR showed that the steady-state message levels were increased in adipose tissue, kidneys, liver and heart in homozygous and heterozygous mice compared to wild-type littermates. Real-time RT-PCR indicated that the transcripts from the knock-in allele were more stable than those from the wild-type allele. (J:154767)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 41 strains or lines available

References

• Original: J:154767 Tsai YS, et al., Genetic variations in peroxisome proliferator-activated receptor gamma expression affect blood pressure. Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19084-9
• All: 2 reference(s)