Igs1^{tm2(CAG-Bgeo,-Ndp,-EGFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Igs1^{tm2(CAG-Bgeo,-Ndp,-EGFP)Nat}
• Name: intergenic site 1; targeted mutation 2, Jeremy Nathans
• MGI ID: MGI:4412094
• Synonyms: Iis1^{tm2(CAG-Bgeo,-Ndp,-EGFP)Nat}, Ubb^tm2Nat, Z/Norrin
• Gene: Igs1 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs1^{tm2(CAG-Bgeo,-Ndp,-EGFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154020
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A site 5' of the Ubb gene was targeted with a construct composed of a CMV/beta-actin enhancer and promoter (CAG), a floxed beta-geo with three transcription termination sites, then Ndb followed by an IRES-EGFP. Cre mediated excision of the floxed beta-geo construct is expected to result in expression of the Ndb/EGFP cassette. (J:154020)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs1 Mutation: 10 strains or lines available

References

• Original: J:154020 Ye X, et al., Norrin, frizzled-4, and Lrp5 signaling in endothelial cells controls a genetic program for retinal vascularization. Cell. 2009 Oct 16;139(2):285-98
• All: 5 reference(s)