Gt(ROSA)26Sor^{tm1.1(CAG-EGFP)Fsh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-EGFP)Fsh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Gordon Fishell
• MGI ID: MGI:4412373
• Synonyms: R26fsGFP, R26^RCE, RCE, RCE^EGFP, RCE^fl, RCE^fsEGFP, RCE-GFP, RCE:loxP, RCE-LoxP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-EGFP)Fsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155164
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The CAG promoter drives expression of EGFP preceded by a floxed neo and STOP cassette and a second frt flanked STOP cassette. In this allele, germ line, flp mediated recombination removes the second STOP cassette leaving a single FRT site. Removal of the first neo/STOP cassette is required to achieve EGFP expression. (J:155164)

Expression

In Mice Carrying this Mutation:

8 assay results 6 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:155164 Sousa VH, et al., Characterization of Nkx6-2-derived neocortical interneuron lineages. Cereb Cortex. 2009 Jul;19 Suppl 1:i1-10
• All: 130 reference(s)