Lgmn^tm1Cptr
Targeted Allele Detail

Summary

• Symbol: Lgmn^tm1Cptr
• Name: legumain; targeted mutation 1, Christoph Peters
• MGI ID: MGI:4415342
• Synonyms: AEP^-
• Gene: Lgmn Location: Chr12:102360341-102405987 bp, - strand Genetic Position: Chr12, 51.45 cM
• Alliance: Lgmn^tm1Cptr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155339
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was replaced by a neomycin cassette via homologous recombination. Absence of expression was verified by RT-PCR, western blot and protease activity using a specific fluorescent substrate. (J:155339)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Lgmn^tm1Cptr/Lgmn^tm1Cptr	B6.129S6-Lgmn^tm1Cptr

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
cellular	√
lysosomal protein accumulation	√
growth/size/body	√
slow postnatal weight gain	√
renal glomerulus cyst	√
enlarged spleen	√
hematopoietic system	√
enlarged spleen	√
homeostasis/metabolism	√
increased circulating creatinine level	√
increased urine protein level	√
albuminuria	√
immune system	√
enlarged spleen	√
abnormal adaptive immunity	√
decreased interleukin-12b secretion	√
decreased interleukin-6 secretion	√
decreased tumor necrosis factor secretion	√
renal/urinary system	√
renal glomerulus cyst	√
increased urine protein level	√
albuminuria	√
abnormal renal glomerulus morphology	√
kidney cortex atrophy	√
abnormal kidney interstitium morphology	√
renal interstitial fibrosis	√
abnormal kidney outer medulla outer stripe morphology	√
hydronephrosis	√
renal tubule atrophy	√
abnormal proximal convoluted tubule morphology	√
pale kidney	√
abnormal kidney physiology	√
decreased renal glomerular filtration rate	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgmn Mutation: 35 strains or lines available

References

• Original: J:155339 Sepulveda FE, et al., Critical role for asparagine endopeptidase in endocytic Toll-like receptor signaling in dendritic cells. Immunity. 2009 Nov 20;31(5):737-48
• All: 7 reference(s)