Tg(CAG-EGFP,RNU6-RNAi:Sly)367Psb
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP,RNU6-RNAi:Sly)367Psb
• Name: transgene insertion 367, Paul S Burgoyne
• MGI ID: MGI:4415346
• Synonyms: sh367
• Transgene: Tg(CAG-EGFP,RNU6-RNAi:Sly)367Psb Location: unknown
• Alliance: Tg(CAG-EGFP,RNU6-RNAi:Sly)367Psb page

Transgene origin

• Strain of Origin: (CBA/Ca x C57BL/10)F1

Transgene description

• Transgene Type: Transgenic (Knockdown, Reporter)
• Mutation: Insertion
• Tg(CAG-EGFP,RNU6-RNAi:Sly)367Psb involves 1 genes/genome features (Sly) View all
• Mutation details: A CAG promoter drive ubiquitous expression of EGFP and the human U6 shRNA promoter (RNU6) drives ubiquitous expression of a short hairpin RNA targeting Sly. Two lines were created (line 136 and 367). Western blot analysis confirmed the absence of endogenous Sly protein expression in both lines. Line 367 was selected as the representative line. (J:155571)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:155571 Cocquet J, et al., The multicopy gene Sly represses the sex chromosomes in the male mouse germline after meiosis. PLoS Biol. 2009 Nov;7(11):e1000244
• All: 6 reference(s)