Chst1^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Chst1^{tm1(KOMP)Vlcg}
• Name: carbohydrate sulfotransferase 1; targeted mutation 1, Velocigene
• MGI ID: MGI:4415360
• Gene: Chst1 Location: Chr2:92430052-92445595 bp, + strand Genetic Position: Chr2, 51.17 cM, cytoband D-E1
• Alliance: Chst1^{tm1(KOMP)Vlcg} page
• IMPC: Chst1 gene page

Mutation origin

• Mutant Cell Lines: 10172A-D6, 10172B-A7, 10172B-D12
• Germline Transmission: Earliest citation of germline transmission: J:204813
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 1285bp between positions 92443516-92444800 of Chromosome 2 (Build GRCm39). The lacZ and floxed neomycin resistance cassette replaced the coding sequence. (J:136110, J:204813)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Chst1 Mutation: 20 strains or lines available

Notes

Cell line 10172A-D6 was successfully used to make chimeric mice. Germline transmission was accomplished. J:204813

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 8 reference(s)