Parp11^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Parp11^{tm1(KOMP)Vlcg}
• Name: poly (ADP-ribose) polymerase family, member 11; targeted mutation 1, Velocigene
• MGI ID: MGI:4415414
• Synonyms: Parp11^-
• Gene: Parp11 Location: Chr6:127423803-127471224 bp, + strand Genetic Position: Chr6, 62.39 cM
• Alliance: Parp11^{tm1(KOMP)Vlcg} page
• IMPC: Parp11 gene page

Mutation origin

• Mutant Cell Lines: 12032A-A10, 12032A-B7, 12032A-D4, 12032A-F12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 21037bp between positions 127447671-127468707 of Chromosome 6 (Build GRCm39). This insertion results in the removal of coding exons 4-8 and allows expression of the reporter gene beta-galactosidase under the control of the endogenous promoter. Semi-quantitative RT-PCR analysis of total RNA confirmed the absence of transcripts in kidney, brain, lung and testis tissues from homozygous mutant mice. (J:136110, J:221906)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Parp11^tm1(KOMP)Vlcg/Parp11^tm1(KOMP)Vlcg	involves: C57BL/6NTac	12032A-B7

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms Sex:
cellular	√
teratozoospermia	√
abnormal sperm head morphology	√
abnormal sperm nucleus morphology	√
abnormal spermatid morphology	√
reproductive system	√
reproductive system phenotype	N
teratozoospermia	√
abnormal sperm head morphology	√
abnormal sperm nucleus morphology	√
abnormal spermatid morphology	√
abnormal spermiogenesis	√
male infertility	√
reduced male fertility	√
decreased fertilization frequency	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Parp11 Mutation: 23 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 5 reference(s)