Lrp6^tm1.2Cjz
Targeted Allele Detail

Summary

• Symbol: Lrp6^tm1.2Cjz
• Name: low density lipoprotein receptor-related protein 6; targeted mutation 1.2, Chengji J Zhou
• MGI ID: MGI:4415718
• Synonyms: Lrp6^floxdel
• Gene: Lrp6 Location: Chr6:134423439-134543876 bp, - strand Genetic Position: Chr6, 65.37 cM
• Alliance: Lrp6^tm1.2Cjz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155250
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed and FRT flanked PGK-neo cassette was inserted in intron2and an additional loxP site was inserted 5' of exon 2. The targeted region, encompassing exon 2, is 1.7 Kb in size. The FRT flanked PGK-neo cassette was excised by crossing to mice carrying the Tg(ACTFLPe)9205Dym transgene. Exon 2 was then deleted by crossing to mice carrying Tg(CMV-cre)1Cgn. Normal mRNA was not detected by Northern blot or RT-PCR. Protein could not be detected by Western blot. (J:155250)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lrp6 Mutation: 95 strains or lines available

References

• Original: J:155250 Zhou CJ, et al., Generation of Lrp6 conditional gene-targeting mouse line for modeling and dissecting multiple birth defects/congenital anomalies. Dev Dyn. 2009 Aug 3;239(1):318-326
• All: 2 reference(s)