Tg(ACTA1-Il15*)11650Lsq
Transgene Detail

Summary

• Symbol: Tg(ACTA1-Il15*)11650Lsq
• Name: transgene insertion 11650, LeBris Smith Quinn
• MGI ID: MGI:4417881
• Synonyms: HSA-IL2SP-IL15
• Transgene: Tg(ACTA1-Il15*)11650Lsq Location: unknown
• Alliance: Tg(ACTA1-Il15*)11650Lsq page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTA1-Il15*)11650Lsq expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Il15 (MGI:103014)

• Mutation details: A mouse interleukin-15 (Il15) cDNA sequence encoding the mature endogenous protein (nucleotides 610-951) was modified by removing several post-transcriptional checkpoints that block efficient IL-15 translation and replacing the inefficiently translated and secreted endogenous IL-15 signal peptides with the mouse interleukin-2 (Il2) signal peptide, and stabilizing the COOH terminus of the mature IL-15 protein with a FLAG epitope tag. This IL2SP-IL15 construct was then placed downstream of a modified human alpha-skeletal actin (HSA or ACTA1) promoter fragment. A polyA signal is inserted at the 3' end of the final transgene. The resulting construct was microinjected into C57BL/6 x C3H hybrid oocyte pronuclei. Founder mice were with C57BL/6J mice to generate line 11650 (with a stable single transgene insertion point). IL-15 mRNA/protein expression in skeletal muscle is approximately 1000-fold greater in hemizygous males and approximately 800-fold/1000-fold greater in hemizygous females, respectively. Overexpression in the serum of hemizygous males and females is approximately 300-fold/500-fold, respectively. (J:145611)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:145611 Quinn LS, et al., Oversecretion of interleukin-15 from skeletal muscle reduces adiposity. Am J Physiol Endocrinol Metab. 2009 Jan;296(1):E191-202
• All: 2 reference(s)