Gt(ROSA)26Sor^{tm1(RNU6-RNAi:Trpv1)Thch}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(RNU6-RNAi:Trpv1)Thch}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Thomas Christoph
• MGI ID: MGI:4418115
• Synonyms: TRPV1 shRNAtg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(RNU6-RNAi:Trpv1)Thch} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132700
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Knockdown, RMCE-ready)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(RNU6-RNAi:Trpv1)Thch} involves 1 genes/genome features (Trpv1) View all
• Mutation details: The siRNA VsiR1 driven by the human U6 (RNU6) promoter was inserted into the locus using recombinase mediated cassette exchange (RMCE). Decreased expression of Trpv1 was confirmed by RT-PCR analysis and reduced protein expression was confirmed by a lack of receptor agonist binding. (J:132700)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:132700 Christoph T, et al., Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice. Mol Cell Neurosci. 2008 Mar;37(3):579-89
• All: 1 reference(s)