Brca1^tm5.1Cxd
Targeted Allele Detail

Summary

• Symbol: Brca1^tm5.1Cxd
• Name: breast cancer 1, early onset; targeted mutation 5.1, Chu-Xia Deng
• MGI ID: MGI:4418211
• Synonyms: Brca1^S1152A
• Gene: Brca1 Location: Chr11:101379590-101442781 bp, - strand Genetic Position: Chr11, 65.18 cM, cytoband D
• Alliance: Brca1^tm5.1Cxd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155810
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A serine to alanine substitution at amino acid 1152 (S1152A) was introduced into exon 11 and a floxed neo cassette was inserted upstream of exon 11 via homologous recombination. Mating with mice expressing cre recombinase in the germ line excised the floxed neo. This allele results in a mutation of the ataxia telangiectasia mutated homolog (ATM) phosphorylation site. Western blot analysis indicated that mutant protein was expressed at levels similar to those of wild type. (J:155810)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca1 Mutation: 114 strains or lines available

References

• Original: J:155810 Kim SS, et al., Impaired skin and mammary gland development and increased gamma-irradiation-induced tumorigenesis in mice carrying a mutation of S1152-ATM phosphorylation site in Brca1. Cancer Res. 2009 Dec 15;69(24):9291-300
• All: 1 reference(s)