Asxl1^tm1Bc
Targeted Allele Detail

Summary

• Symbol: Asxl1^tm1Bc
• Name: ASXL transcriptional regulator 1; targeted mutation 1, University of British Columbia
• MGI ID: MGI:4418244
• Gene: Asxl1 Location: Chr2:153187750-153245927 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Asxl1^tm1Bc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155898
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-neomycin resistance cassette was inserted in reverse orientation into exon 5 upstream of the conserved ASXH and PHD domains. The insertion interrupts the reading frame at codon 90 by the introduction of several premature termination codons. Northern blot analysis confirmed that insertion occurred as expected. (J:155898)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asxl1 Mutation: 116 strains or lines available

References

• Original: J:155898 Fisher CL, et al., Loss-of-function Additional sex combs like 1 mutations disrupt hematopoiesis but do not cause severe myelodysplasia or leukemia. Blood. 2010 Jan 7;115(1):38-46
• All: 2 reference(s)