Tg(Col2a1-rtTA,tetO-cre)22Pjro
Transgene Detail

Summary

• Symbol: Tg(Col2a1-rtTA,tetO-cre)22Pjro
• Name: transgene insertion 22, Peter J Roughley
• MGI ID: MGI:4418267
• Synonyms: COL2-rtTA-Cre, Tg(Col2a1-rtTA-tetO-cre)22Pjro
• Transgene: Tg(Col2a1-rtTA,tetO-cre)22Pjro Location: unknown
• Alliance: Tg(Col2a1-rtTA,tetO-cre)22Pjro page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Col2a1-rtTA,tetO-cre)22Pjro expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Col2a1

• Mutation details: The construct was generated such that a 6 kb fragment of the Col2a1 gene (composed of 3 kb of promoter and 3 kb of intron 1 containing an enhancer sequence) controls the reverse tetracycline transactivator sequence. A tetracycline responsive element heptad sequence (tetO) with a CMV minimal promoter is upstream of the cre recombinase coding sequence in this construct. Cre expression can only occur by transactivation of rtTA by doxycycline. The linearized construct was injected into fertilized oocytes and founder mice were obtained (lines 13, 12, 22, and 24). Cre mRNA expression was detected only in cartilage, not in the brain, liver or skin of embryos exposed to doxycycline. (J:107728)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Col2a1 (species not specified) Summary of all recombinase alleles driven by Col2a1. Comparative matrix view of recombinase activities

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:107728 Grover J, et al., Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. Matrix Biol. 2006 Apr;25(3):158-65
• All: 6 reference(s)