Phox2b^tm4Jbr
Targeted Allele Detail

Summary

• Symbol: Phox2b^tm4Jbr
• Name: paired-like homeobox 2b; targeted mutation 4, Jean-Francois Brunet
• MGI ID: MGI:4418298
• Synonyms: Phox2b^27Alacki
• Gene: Phox2b Location: Chr5:67251751-67256399 bp, - strand Genetic Position: Chr5, 35.95 cM
• Alliance: Phox2b^tm4Jbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155885
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site and FRT flanked neo cassette were inserted upstream of exon 3 and a loxP site and mutated exon 3 containing a 7 alanine expansion of a 20-residue polyalanine chain were inserted downstream of exon 3 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:155885, J:176573)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Phox2b Mutation: 25 strains or lines available

References

• Original: J:155885 Dubreuil V, et al., Defective respiratory rhythmogenesis and loss of central chemosensitivity in Phox2b mutants targeting retrotrapezoid nucleus neurons. J Neurosci. 2009 Nov 25;29(47):14836-46
• All: 3 reference(s)