Gad2^tm2(cre)Zjh
Targeted Allele Detail

Summary

• Symbol: Gad2^tm2(cre)Zjh
• Name: glutamic acid decarboxylase 2; targeted mutation 2, Z Josh Huang
• MGI ID: MGI:4418713
• Synonyms: Gad2^Cre, Gad2-iCre, Gad2-ires-Cre, GAD65-Cre, GAD65-iCre, GAD65-ires-Cre
• Gene: Gad2 Location: Chr2:22512262-22583889 bp, + strand Genetic Position: Chr2, 15.15 cM, cytoband A-B
• Alliance: Gad2^tm2(cre)Zjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gad2^tm2(cre)Zjh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gad2 (MGI:95634)

• Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the selection cassette. The FLPe transgene was subsequently bred out of the background. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gad2 (mouse) Summary of all recombinase alleles driven by Gad2. Comparative matrix view of recombinase activities Alliance mouse Gad2 gene page

Expression

In Mice Carrying this Mutation:

12 assay results 4 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Gad2 Mutation: 60 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 356 reference(s)