Sox17^{tm2.1(icre)Heli}
Targeted Allele Detail

Summary

• Symbol: Sox17^{tm2.1(icre)Heli}
• Name: SRY (sex determining region Y)-box 17; targeted mutation 2.1, Heiko Lickert
• MGI ID: MGI:4418897
• Synonyms: Sox17- 2Aicre, Sox17-2A-iCre, Sox17^2A-iCre, Sox17^{2A-iCredeltaneo}, Sox17^{tm2.1(icre)Lic}
• Gene: Sox17 Location: Chr1:4561154-4567577 bp, - strand Genetic Position: Chr1, 1.65 cM
• Alliance: Sox17^{tm2.1(icre)Heli} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155991
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Sox17^{tm2.1(icre)Heli} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sox17 (MGI:107543)

• Mutation details: The Sox17 translational stop codon in exon 5 was replaced by a T2A (Thosea asigna virus, TaV) self-cleaving peptide followed by the improved Cre (iCre) for the efficient and equimolar translation of Sox17 and icre. Sox17 and icre are postranslationally cleaved by a 2A-mediated ribosome-skipping mechanism. The FRT-flanked neo selection cassette was subsequently deleted in the germline by FLP-mediated recombination. (J:155991)

Generation of the Sox17^{tm2.1(icre)Heli} allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sox17 (mouse) Summary of all recombinase alleles driven by Sox17. Comparative matrix view of recombinase activities Alliance mouse Sox17 gene page

Expression

In Mice Carrying this Mutation:

28 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox17 Mutation: 29 strains or lines available

References

• Original: J:155991 Engert S, et al., Sox17-2A-iCre: a knock-in mouse line expressing Cre recombinase in endoderm and vascular endothelial cells. Genesis. 2009 Sep;47(9):603-10
• All: 18 reference(s)