Ciart^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ciart^{tm1e(KOMP)Wtsi}
• Name: circadian associated repressor of transcription; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419228
• Synonyms: Gm129^-
• Gene: Ciart Location: Chr3:95785815-95789563 bp, - strand Genetic Position: Chr3, 41.42 cM
• Alliance: Ciart^{tm1e(KOMP)Wtsi} page
• IMPC: Ciart gene page

Mutation origin

• Mutant Cell Lines: EPD0378_5_A04, EPD0378_5_B02, EPD0378_5_B04, EPD0378_5_C01, EPD0378_5_C02, EPD0378_5_C03, EPD0378_5_C04, EPD0378_5_D01, EPD0378_5_D03, EPD0378_5_D04, EPD0378_5_E01, EPD0378_5_E02, EPD0378_5_E04, EPD0378_5_F01, EPD0378_5_F02, EPD0378_5_F03, EPD0378_5_G01, EPD0378_5_G02, EPD0378_5_H01, EPD0378_5_H02, EPD0378_5_H04
• Germline Transmission: Earliest citation of germline transmission: J:205586
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95789196 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:205586, J:208319)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ciart Mutation: 27 strains or lines available

Notes

Cell line EPD0378_5_G02 was successfully used to make chimeric mice. Germline transmission was accomplished. J: 205586

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)