Rec114^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rec114^{tm1(KOMP)Wtsi}
• Name: REC114 meiotic recombination protein; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419253
• Synonyms: 2410076I21Rik^{tm1(KOMP)Wtsi}, Rec114^-
• Gene: Rec114 Location: Chr9:58560133-58651247 bp, - strand Genetic Position: Chr9, 31.79 cM, cytoband C
• Alliance: Rec114^{tm1(KOMP)Wtsi} page
• IMPC: Rec114 gene page

Mutation origin

• Mutant Cell Lines: EPD0392_4_D08, EPD0392_4_E07, EPD0392_4_E08, EPD0392_4_F06, EPD0392_4_F08, EPD0392_4_G06, EPD0392_4_G08, EPD0392_4_H05, EPD0392_4_H06, EPD0392_4_H07
• Germline Transmission: Earliest citation of germline transmission: J:271216
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 5762 starting at position 58567450 and ending at position 58573212 of Chromosome 9 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534, J:271216)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Rec114 Mutation: 14 strains or lines available

Notes

Cell line EPD0392_4_D08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:271216

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)