Arhgef3^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arhgef3^{tm1(KOMP)Wtsi}
• Name: Rho guanine nucleotide exchange factor 3; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419295
• Gene: Arhgef3 Location: Chr14:26836856-27125868 bp, + strand Genetic Position: Chr14, 16.09 cM
• Alliance: Arhgef3^{tm1(KOMP)Wtsi} page
• IMPC: Arhgef3 gene page

Mutation origin

• Mutant Cell Lines: EPD0416_7_A05, EPD0416_7_A06, EPD0416_7_B06, EPD0416_7_B07, EPD0416_7_B08, EPD0416_7_C05, EPD0416_7_C06, EPD0416_7_C07, EPD0416_7_C08, EPD0416_7_D05, EPD0416_7_D08, EPD0416_7_E05, EPD0416_7_E06, EPD0416_7_F05, EPD0416_7_F07, EPD0416_7_G05, EPD0416_7_G08, EPD0416_7_H05, EPD0416_7_H06, EPD0416_7_H07, EPD0416_7_H08
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2098 starting at position 27106077 and ending at position 27108175 of Chromosome 14 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534, J:241286)

Generation of the Arhgef3^tm1(KOMP)Wtsi allele

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Arhgef3 Mutation: 96 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)