Bola2^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bola2^{tm1(KOMP)Wtsi}
• Name: bolA family member 2; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419429
• Gene: Bola2 Location: Chr7:126295172-126295865 bp, + strand Genetic Position: Chr7, 69.25 cM
• Alliance: Bola2^{tm1(KOMP)Wtsi} page
• IMPC: Bola2 gene page

Mutation origin

• Mutant Cell Lines: EPD0384_6_A09, EPD0384_6_A10, EPD0384_6_A11, EPD0384_6_B10, EPD0384_6_C10, EPD0384_6_C11, EPD0384_6_D11, EPD0384_6_D12, EPD0384_6_E11, EPD0384_6_E12, EPD0384_6_F09, EPD0384_6_F10, EPD0384_6_F11, EPD0384_6_G10, EPD0384_6_G11, EPD0384_6_G12, EPD0384_6_H11, EPD0384_6_H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 564 starting at position 126295347 and ending at position 126295911 of Chromosome 7 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Bola2 Mutation: 5 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 7 reference(s)