Ankrd65^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ankrd65^{tm1(KOMP)Wtsi}
• Name: ankyrin repeat domain 65; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419461
• Gene: Ankrd65 Location: Chr4:155874896-155877659 bp, + strand Genetic Position: Chr4, 87.39 cM
• Alliance: Ankrd65^{tm1(KOMP)Wtsi} page
• IMPC: Ankrd65 gene page

Mutation origin

• Mutant Cell Lines: EPD0412_4_A05, EPD0412_4_B07, EPD0412_4_B08, EPD0412_4_C05, EPD0412_4_C07, EPD0412_4_E05, EPD0412_4_E06, EPD0412_4_E07, EPD0412_4_F05, EPD0412_4_G05, EPD0412_4_G07
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 582 starting at position 155876033 and ending at position 155876615 of Chromosome 4 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ankrd65 Mutation: 11 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)