Tubb4a^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tubb4a^{tm1(KOMP)Wtsi}
• Name: tubulin, beta 4A class IVA; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419785
• Synonyms: Tubb4a^null
• Gene: Tubb4a Location: Chr17:57387061-57394600 bp, - strand Genetic Position: Chr17, 29.66 cM
• Alliance: Tubb4a^{tm1(KOMP)Wtsi} page
• IMPC: Tubb4a gene page

Mutation origin

• Mutant Cell Lines: EPD0391_2_A06, EPD0391_2_C05, EPD0391_2_C08, EPD0391_2_D05, EPD0391_2_D07, EPD0391_2_D08, EPD0391_2_E05, EPD0391_2_F05, EPD0391_2_F07, EPD0391_2_F08, EPD0391_2_H06, EPD0391_2_H08
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 5753 starting at position 57387655 and ending at position 57393408 of Chromosome 17 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Tubb4a Mutation: 30 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)