Nexmif^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nexmif^{tm1(KOMP)Wtsi}
• Name: neurite extension and migration factor; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419911
• Synonyms: C77370^{tm1(KOMP)Wtsi}
• Gene: Nexmif Location: ChrX:103121040-103244791 bp, - strand Genetic Position: ChrX, 46.48 cM
• Alliance: Nexmif^{tm1(KOMP)Wtsi} page
• IMPC: Nexmif gene page

Mutation origin

• Mutant Cell Lines: EPD0411_7_B01, EPD0411_7_B02, EPD0411_7_H02
• Germline Transmission: Earliest citation of germline transmission: J:283395
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 4479 starting at position 103127410 and ending at position 103131889 of Chromosome X (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534, J:283395)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Nexmif Mutation: 55 strains or lines available

Notes

Cell line EPD0411_7_H02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:283395.

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)