H2-M5^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: H2-M5^{tm1(KOMP)Wtsi}
• Name: histocompatibility 2, M region locus 5; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4420089
• Gene: H2-M5 Location: Chr17:37294953-37300429 bp, - strand Genetic Position: Chr17, 19.16 cM
• Alliance: H2-M5^{tm1(KOMP)Wtsi} page
• IMPC: H2-M5 gene page

Mutation origin

• Mutant Cell Line: EPD0406_1_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2097 starting at position 37298090 and ending at position 37300187 of Chromosome 17 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any H2-M5 Mutation: 18 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)