Tg(Lfng*-LFNG)1Dihz
Transgene Detail

Summary

• Symbol: Tg(Lfng*-LFNG)1Dihz
• Name: transgene insertion 1, David Ish-Horowicz
• MGI ID: MGI:4420243
• Synonyms: BB-cLfng, BBL-1
• Transgene: Tg(Lfng*-LFNG)1Dihz Location: unknown
• Alliance: Tg(Lfng*-LFNG)1Dihz page

Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain

Show the 3 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (CBA x C57BL/6J)

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Lfng*-LFNG)1Dihz expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  chicken	LFNG (395790)

• Mutation details: This transgene consists of a tandem duplication of the 285 bp B-block/block 3 enhancer of the mouse Lfng gene, the beta globin basal promoter, the open reading frame encoding chicken LFNG with a C-terminal FLAG tag, and the SV40 3' UTR and polyadenylation signal. The Lfng B-block enhancer drives expression in the cranial presomitic mesoderm (PSM) stripe adjacent to the incipient somite border. Thirteen lines were generated. This line expresses the transgene in the cranial stripe and only very weakly in the caudal presomitic mesoderm. (J:155378)

Schematic of the Tg(Lfng*-LFNG)1Dihz, Tg(Lfng*-LFNG)2Dihz, Tg(Lfng-LFNG)1Dihz and Tg(Lfng-LFNG)2Dihz transgenes

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:155378 Stauber M, et al., Differential axial requirements for lunatic fringe and Hes7 transcription during mouse somitogenesis. PLoS One. 2009;4(11):e7996
• All: 1 reference(s)