Atoh1^tm6.1Hzo
Targeted Allele Detail

Summary

• Symbol: Atoh1^tm6.1Hzo
• Name: atonal bHLH transcription factor 1; targeted mutation 6.1, Huda Y Zoghbi
• MGI ID: MGI:4420925
• Synonyms: Atoh1^flag
• Gene: Atoh1 Location: Chr6:64706109-64708229 bp, + strand Genetic Position: Chr6, 30.03 cM
• Alliance: Atoh1^tm6.1Hzo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155047
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, No functional change, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cDNA encoding for a version of the gene with three C-terminal FLAG tags and an FRT-NEO-FRT selection cassette was inserted, replacing the entire coding sequence. The selection cassette was then removed by flp mediated recombination. Expression of the tagged protein in the cerebellum but not the cortex was confirmed by chromatin immunoprecipitation analysis. (J:155047)

Expression

In Mice Carrying this Mutation:

21 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atoh1 Mutation: 37 strains or lines available

References

• Original: J:155047 Flora A, et al., Deletion of Atoh1 disrupts Sonic Hedgehog signaling in the developing cerebellum and prevents medulloblastoma. Science. 2009 Dec 4;326(5958):1424-7
• All: 5 reference(s)