Gjc3^tm1.1Kwi
Targeted Allele Detail

Summary

• Symbol: Gjc3^tm1.1Kwi
• Name: gap junction protein, gamma 3; targeted mutation 1.1, Klaus Willecke
• MGI ID: MGI:4420945
• Synonyms: Cx29^lacZ
• Gene: Gjc3 Location: Chr5:137951723-137961360 bp, - strand Genetic Position: Chr5, 76.91 cM
• Alliance: Gjc3^tm1.1Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156133
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous locus was replaced with an nls-lacZ-SV40 poly(A) gene, leaving the original splice acceptor site and translational start in place, as well as the 3' homologous region right after the TAG stop codon, unaltered. An FRT flanked HPRT gene was placed in front of the 3' homologous region in the reverse orientation. The HPRT gene was removed by Flp mediated recombination. Western blot analysis confirmed the absence of protein expression in the brains of homozygous mice. LacZ expression was confirmed in Schwann cells, oligodendrocytes and Bergmann glia cells. (J:156133)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gjc3 Mutation: 15 strains or lines available

References

• Original: J:156133 Eiberger J, et al., Expression pattern and functional characterization of connexin29 in transgenic mice. Glia. 2006 Apr 15;53(6):601-11
• All: 3 reference(s)