Bmf^tm2.1Rjd
Targeted Allele Detail

Summary

• Symbol: Bmf^tm2.1Rjd
• Name: BCL2 modifying factor; targeted mutation 2.1, Roger J Davis
• MGI ID: MGI:4421179
• Synonyms: Bmf^S74D, Bmf^SD
• Gene: Bmf Location: Chr2:118359238-118380168 bp, - strand Genetic Position: Chr2, 59.4 cM, cytoband E5
• Alliance: Bmf^tm2.1Rjd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153652
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A floxed neo cassette was inserted within intron 3, and specific nucleotide mutations in exon 3 were introduced by homologous recombination. Mutation resulted in the JNK protein kinase phosphorylation site (Ser-74) in exon 3 to be substituted with an Asp (S74D). The neo cassette was then excised with Cre mediated recombination leaving a single loxP site in intron 3. (J:153652)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bmf Mutation: 21 strains or lines available

References

• Original: J:153652 Hubner A, et al., Functional cooperation of the proapoptotic Bcl2 family proteins Bmf and Bim in vivo. Mol Cell Biol. 2010 Jan;30(1):98-105
• All: 2 reference(s)