Tg(Camk2a-cre/ERT2)1Aibs
Transgene Detail

Summary

• Symbol: Tg(Camk2a-cre/ERT2)1Aibs
• Name: transgene insertion 1, Allen Institute for Brain Science
• MGI ID: MGI:4421787
• Synonyms: Camk2a-CreERT2
• Transgene: Tg(Camk2a-cre/ERT2)1Aibs Location: unknown
• Alliance: Tg(Camk2a-cre/ERT2)1Aibs page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:155793
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Camk2a-cre/ERT2)1Aibs expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Camk2a (MGI:88256)

• Mutation details: The Camk2a-CreERT2 transgene was designed with the mouse Camk2a promoter, CreERT2 fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), bovine growth hormone polyA signal, and PGK-Puro-SV40polyA cassette. (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells, already targeted with an Ai3 mutation in the Gt(ROSA)26Sor locus, were transfected with the Camk2a-CreERT2 transgene. Correctly targeted ES cells harboring both the Ai3 mutation and Camk2a-CreERT2 transgene were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6J mice to generate the double mutant colony. Subsequent mating with C57BL/6J wildtype mice resulted in offspring harboring only the Camk2a-CreERT2 transgene (wildtype at the Gt(ROSA)26Sor locus). (J:155793)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Camk2a (mouse) Summary of all recombinase alleles driven by Camk2a. Comparative matrix view of recombinase activities Alliance mouse Camk2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
• All: 55 reference(s)