Erbb4^{tm1.1(cre/ERT2)Aibs}
Targeted Allele Detail

Summary

• Symbol: Erbb4^{tm1.1(cre/ERT2)Aibs}
• Name: erb-b2 receptor tyrosine kinase 4; targeted mutation 1.1, Allen Institute for Brain Science
• MGI ID: MGI:4421788
• Synonyms: Erbb4-2A-CreERT2, Erbb4^{tm1(cre/ERT2)Aibs}
• Gene: Erbb4 Location: Chr1:68071345-69147218 bp, - strand Genetic Position: Chr1, 33.8 cM
• Alliance: Erbb4^{tm1.1(cre/ERT2)Aibs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155793
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Erbb4^{tm1.1(cre/ERT2)Aibs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Erbb4 (MGI:104771)

• Mutation details: A targeting vector was designed to insert a viral 2A oligopeptide that mediates ribosomal skipping and a CreERT2 fusion gene immediately downstream of the translational STOP codon of the Erbb4 locus (v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)). The targeting vector contained, from 5' to 3', an frt3 site, a viral 2A oligopeptide, a CreERT2 fusion gene, a bovine growth hormone polyA signal, an attB site, a PGK-Neo-polyA cassette, an frt5 site, an RNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an attP site. This construct was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6J mice to generate the Erbb4-2A-CreERT2 mutant colony. These mutant mice were bred to PhiC31-expressing mice (C57BL/6J congenic background) to remove the PGK-Neo-polyA cassette, frt5 site, RNA splice acceptor, and the 3' portion of the hygromycin gene with SV40 polyA signal. Following tamoxifen induction, Erbb4-2A-CreERT2 directs reporter gene expression in scattered interneuron subpopulations of Erbb4-expressing cells in the cortex and hippocampus. (J:155793)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Erbb4 (mouse) Summary of all recombinase alleles driven by Erbb4. Comparative matrix view of recombinase activities Alliance mouse Erbb4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Erbb4 Mutation: 87 strains or lines available

References

• Original: J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
• All: 21 reference(s)