Tdrd9^tm1.1Chum
Targeted Allele Detail

Summary

• Symbol: Tdrd9^tm1.1Chum
• Name: tudor domain containing 9; targeted mutation 1.1, Shinichiro Chuma
• MGI ID: MGI:4421849
• Synonyms: Tdrd9^-
• Gene: Tdrd9 Location: Chr12:111937993-112035288 bp, + strand Genetic Position: Chr12, 61.14 cM, cytoband F2
• Alliance: Tdrd9^tm1.1Chum page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156018
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3, and a pgk-neo cassette flanked by FRT sites as well as a second loxP site were targeted downstream of exon 7. Chimeric mice were produced from two recombinant ES cell clones. Exons 3-7 were excised by Cre mediated recombination. Western blot, immunostaining and RT-PCR showed no protein or RNA were detectable in the mutant testis. (J:156018)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tdrd9 Mutation: 73 strains or lines available

References

• Original: J:156018 Shoji M, et al., The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. Dev Cell. 2009 Dec;17(6):775-87
• All: 3 reference(s)