Ewsr1^{tm2(FLI1*)Sblee}
Targeted Allele Detail

Summary

• Symbol: Ewsr1^{tm2(FLI1*)Sblee}
• Name: Ewing sarcoma breakpoint region 1; targeted mutation 2, Sean Lee
• MGI ID: MGI:4429147
• Synonyms: EF^Stop, Ews/FL1^Stop
• Gene: Ewsr1 Location: Chr11:5019689-5049266 bp, - strand Genetic Position: Chr11, 3.22 cM, cytoband A1-A3
• Alliance: Ewsr1^{tm2(FLI1*)Sblee} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156860
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Ewsr1^{tm2(FLI1*)Sblee} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FLI1 (2313)

  Fli1	(MGI:95554)

• Mutation details: A loxP-flanked transcriptional stop signal in the antisense direction was inserted into intron 6. Exon 7 was fused to exons 5-9 of human FLI1 with a C-terminal FLAG tag, and a 3' neo cassette, replacing exons 8 through 11 by homologous recombination. The absence of endogenous protein product was confirmed by western blot analysis. (J:156860)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ewsr1 Mutation: 93 strains or lines available

References

• Original: J:156860 Sohn EJ, et al., EWS/FLI1 oncogene activates caspase 3 transcription and triggers apoptosis in vivo. Cancer Res. 2010 Feb 1;70(3):1154-63
• All: 2 reference(s)