Mcph1^tm1.2Kali
Targeted Allele Detail

Summary

• Symbol: Mcph1^tm1.2Kali
• Name: microcephaly, primary autosomal recessive 1; targeted mutation 1.2, Kaiyi Li
• MGI ID: MGI:4429492
• Synonyms: BRIT1^-
• Gene: Mcph1 Location: Chr8:18645147-18853205 bp, + strand Genetic Position: Chr8, 10.24 cM, cytoband A3
• Alliance: Mcph1^tm1.2Kali page

Mcph1^tm1.2Kali/Mcph1^tm1.2Kali males are infertile and exhibit meiotic defects

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156371
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exon 2 was floxed and a FRT flanked neo cassette was added within intron 2 by homologous recombination. The neo cassette was removed by flp mediated recombination. Exon 2 was removed by cre mediated recombination. Protein loss was confirmed by western blot. (J:156371)

Generation of the Mcph1^tm1.2Kali allele

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcph1 Mutation: 58 strains or lines available

References

• Original: J:156371 Liang Y, et al., BRIT1/MCPH1 is essential for mitotic and meiotic recombination DNA repair and maintaining genomic stability in mice. PLoS Genet. 2010 Jan;6(1):e1000826
• All: 4 reference(s)