Rfx6^{tm1.1(EGFP/cre)Mger}
Targeted Allele Detail

Summary

• Symbol: Rfx6^{tm1.1(EGFP/cre)Mger}
• Name: regulatory factor X, 6; targeted mutation 1.1, Michael S German
• MGI ID: MGI:4429702
• Synonyms: Rfx6^eGFPcre
• Gene: Rfx6 Location: Chr10:51553856-51606525 bp, + strand Genetic Position: Chr10, 25.74 cM
• Alliance: Rfx6^{tm1.1(EGFP/cre)Mger} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156925
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Rfx6^{tm1.1(EGFP/cre)Mger} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rfx6 (MGI:2445208)

• Mutation details: Recombineering methods were used to modify a BAC containing the Rfx6 gene and generate the targeting construct. Homologous recombination in ES cells resulted in the first 5 exons being replaced by and EFGP/cre fusion gene followed by an FRT-flanked neo cassette. The neo was subsequently removed by crossing mice carrying the disrupted allele with Actb-Flp mice. (J:156925)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rfx6 (mouse) Summary of all recombinase alleles driven by Rfx6. Alliance mouse Rfx6 gene page

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rfx6 Mutation: 52 strains or lines available

References

• Original: J:156925 Smith SB, et al., Rfx6 directs islet formation and insulin production in mice and humans. Nature. 2010 Feb 11;463(7282):775-80
• All: 5 reference(s)