Tg(Foxp3-EGFP/icre)1aJbs
Transgene Detail

Summary

• Symbol: Tg(Foxp3-EGFP/icre)1aJbs
• Name: transgene insertion 1a, Jeffrey Bluestone
• MGI ID: MGI:4430213
• Transgene: Tg(Foxp3-EGFP/icre)1aJbs Location: unknown
• Alliance: Tg(Foxp3-EGFP/icre)1aJbs page

Transgene origin

• Strain of Origin: NOD/ShiLtJ

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Foxp3-EGFP/icre)1aJbs expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxp3 (MGI:1891436)

• Mutation details: An EGFP-h-Cre cDNA (GFP fused to a codon-optimized, "humanized" cre recombinase sequence) was inserted directly downstream of the Foxp3 ATG start codon by homologous recombination in a 188-kb C57BL/6 BAC clone (RP23-143D8) containing the entire Foxp3 gene. The purified BAC was injected into pronuclei of nonobese, diabetic mouse embryos. Several founders were generated. GFP expression and lack of functional Foxp3 protein, as indicated by inability of the transgene to rescue Foxp3 null mice were analyzed and line 1 was established. Functional cre activity was assayed by breeding mice to Gt(ROSA)26Sor^tm1(EYFP)Cos reporter mice, and high specificity of recombination was detected in the lymph nodes and spleen. This is the first of two lines derived from founder 1. Cre mediated recombination and GFP expression is greater than in line 1c. (J:138808)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxp3 (mouse) Summary of all recombinase alleles driven by Foxp3. Alliance mouse Foxp3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:138808 Zhou X, et al., Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity. J Exp Med. 2008 Sep 1;205(9):1983-91
• All: 17 reference(s)