Foxp3^tm5.2Ayr
Targeted Allele Detail

Summary

• Symbol: Foxp3^tm5.2Ayr
• Name: forkhead box P3; targeted mutation 5.2, Alexander Y Rudensky
• MGI ID: MGI:4430232
• Synonyms: Foxp3^deltaCNS1gfp
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^tm5.2Ayr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156942
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two loxP sequences flanking conserved non-coding sequence 1 (CNS1 (Rr123)), in intron 1, as well as an FRT site flanked neomycin resistance gene cassette were inserted by homologous recombination. In addition, an EGFP cassette was inserted in-frame into the first coding exon. The selection cassette and the CNS were removed by flp- and cre-mediated recombination. (J:156942)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 58 strains or lines available

References

• Original: J:156942 Zheng Y, et al., Role of conserved non-coding DNA elements in the Foxp3 gene in regulatory T-cell fate. Nature. 2010 Feb 11;463(7282):808-12
• All: 9 reference(s)