Acacb^tm1.1Dejs
Targeted Allele Detail

Summary

• Symbol: Acacb^tm1.1Dejs
• Name: acetyl-Coenzyme A carboxylase beta; targeted mutation 1.1, David James
• MGI ID: MGI:4430244
• Synonyms: ACC2 KO
• Gene: Acacb Location: Chr5:114284748-114388822 bp, + strand Genetic Position: Chr5, 55.97 cM
• Alliance: Acacb^tm1.1Dejs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157000
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon12 was flanked with loxP sites and a neomycin cassette was added. Cre-mediated recombination subsequently removed exon 12 and the neomycin selection cassette and caused a frameshift and an early stop codon. Mice were confirmed deficient by western blotting. (J:157000)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acacb Mutation: 109 strains or lines available

References

• Original: J:157000 Hoehn KL, et al., Acute or chronic upregulation of mitochondrial fatty acid oxidation has no net effect on whole-body energy expenditure or adiposity. Cell Metab. 2010 Jan;11(1):70-6
• All: 5 reference(s)