Fgf8^tm1Jyhl
Targeted Allele Detail

Summary

• Symbol: Fgf8^tm1Jyhl
• Name: fibroblast growth factor 8; targeted mutation 1, James Y H Li
• MGI ID: MGI:4430252
• Synonyms: Fgf8^deltab-neo
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^tm1Jyhl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135128
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The intron-exon junction sequence of exon 1D, taaagGTA was changed to catatGTA, abolishing the 5' alternative splice acceptor, while the downstream alternative splice acceptor in exon 1D remained intact. A neo cassette flanked with two loxP sites was placed 400 bp downstream of exon 1D. RT-PCR analyses revealed that Fgf8b and three minor b-type splice variants were absent in E7.5 mutant embryos. The insertion of neo produced an additional transcript resulted from a cryptic splice donor and acceptor in the neo gene and in the intronic region 360 bp downstream to the neo gene. (J:135128)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 21 strains or lines available

References

• Original: J:135128 Guo Q, et al., Distinct functions of the major Fgf8 spliceform, Fgf8b, before and during mouse gastrulation. Development. 2007 Jun;134(12):2251-60
• All: 1 reference(s)