Epha4^tm1.2Bzh
Targeted Allele Detail

Summary

• Symbol: Epha4^tm1.2Bzh
• Name: Eph receptor A4; targeted mutation 1.2, Binhai Zheng
• MGI ID: MGI:4430286
• Synonyms: EphA4^mCFPNeoNull
• Gene: Epha4 Location: Chr1:77343822-77491725 bp, - strand Genetic Position: Chr1, 39.55 cM, cytoband C1-C5
• Alliance: Epha4^tm1.2Bzh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157159
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: Two loxP sites were inserted flanking exon 3. Preceding the floxed exon 3, a reporter/selection cassette (SA-mCFP-Neo) flanked by two Frt sites, containing a splice acceptor, a membrane-targeted cyan fluorescent protein reporter gene and a neo cassette, was inserted. After Cre-mediated excision of exon 3, a frameshift in the downstream coding sequence resulted in a null allele and a functional mCFP reporter. Western blot analysis on total brain extracts and immunostaining on adult brain tissue confirmed the absence of the protein in homozygous mutants. Immunohistochemistry also confirmed mCFP expression in the hippocampus. (J:157159)

Generation of the Epha4^tm1Bzh, Epha4^tm1.1Bzh and Epha4^tm1.2Bzh alleles

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epha4 Mutation: 67 strains or lines available

References

• Original: J:157159 Herrmann JE, et al., Generation of an EphA4 conditional allele in mice. Genesis. 2010 Feb;48(2):101-5
• All: 4 reference(s)