Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Klaus Rajewsky
• MGI ID: MGI:4430550
• Synonyms: MEk1DD, R26Stop^FLMEK1DD, R26Stop flox MEK1DD, R26Stop-flox-MEK1DD, ROSA^MEK1*
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157297
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Map2k1 (170851)

  Map2k1

  (MGI:1346866)

• Mutation details: A targeting vector designed with (from 5' to 3') a splice acceptor site, a loxP-flanked Neo-STOP cassette, a cDNA sequence encoding rat MEK1DD (a mutant form of MAPKK1 rendered constitutively active by two serine to aspartic acid substitutions (S218D/S222D) within the catalytic domain], an FRT-flanked internal ribosomal entry site-enhanced green fluorescent protein cassette (IRES-EGFP), and bovine polyadenylation signal. This transgene construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:157297)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:157297 Srinivasan L, et al., PI3 kinase signals BCR-dependent mature B cell survival. Cell. 2009 Oct 30;139(3):573-86
• All: 33 reference(s)