Col1a1^{tm1(CAG-Sirt1)Dsin}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(CAG-Sirt1)Dsin}
• Name: collagen, type I, alpha 1; targeted mutation 1, David Sinclair
• MGI ID: MGI:4430570
• Synonyms: Col1a1^{Sirt1-Tg flox-STOP-flox}, ColA1^{flox-STOP-Sirt1}, SIRT1^STOP
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(CAG-Sirt1)Dsin} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134301
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Col1a1^{tm1(CAG-Sirt1)Dsin} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sirt1 (MGI:2135607)

• Mutation details: The SIRT1-Tg flox-STOP-flox construct was designed to insert (from 5' to 3') an FRT site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked transcriptional STOP cassette, the mouse sirtuin 1 (Sirt1) cDNA sequence, and a PGK-hygro-polyA sequence (with a second FRT site between the PGK and hygro) into the 3' UTR of the mouse collagen A1 (Col1a1) locus. (J:134301)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:134301 Firestein R, et al., The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth. PLoS One. 2008;3(4):e2020
• All: 18 reference(s)