Col1a1^{tm5(tetO-Pou5f1,-Klf4,-Myc)Jae}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm5(tetO-Pou5f1,-Klf4,-Myc)Jae}
• Name: collagen, type I, alpha 1; targeted mutation 5, Rudolf Jaenisch
• MGI ID: MGI:4430600
• Synonyms: Col1a1 3F2A, Col1a1-3F2A, Col1a1^3F2A, Col1a1^tetO-3F2A, Col1a1::tetO-3F2A
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm5(tetO-Pou5f1,-Klf4,-Myc)Jae} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157298
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm5(tetO-Pou5f1,-Klf4,-Myc)Jae} expresses 4 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Klf4 (MGI:1342287)
  mouse	Myc (MGI:97250)
  mouse	Pou5f1 (MGI:101893)
  mouse	Sox2 (MGI:98364)

• Mutation details: RMCE on KH2 ES cells inserted a cassette containing (from 5' to 3') a loxP-flanked pgkNEOpolyA cassette, the tetracycline responsive element (TRE or tetO), the single polycistronic expression cassette 3F2A, and SV40 intron plus polyA signal into the 3' UTR. The 3F2A cassette contains the three mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Klf4 (Kruppel-like factor 4 (gut)), and c-Myc (Myc; myelocytomatosis oncogene) separated by two different viral 2A oligopeptides that mediate ribosomal skipping (T2A [from insect Thosea asigna virus] and E2A [equine rhinitis A virus], respectively). (J:157298)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:157298 Carey BW, et al., Single-gene transgenic mouse strains for reprogramming adult somatic cells. Nat Methods. 2010 Jan;7(1):56-9
• All: 1 reference(s)