Vip^tm1(cre)Zjh
Targeted Allele Detail

Summary

• Symbol: Vip^tm1(cre)Zjh
• Name: vasoactive intestinal polypeptide; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:4431361
• Synonyms: VIP-Cre, Vip-IRES-cre
• Gene: Vip Location: Chr10:5589218-5597617 bp, + strand Genetic Position: Chr10, 1.97 cM, cytoband A1
• Alliance: Vip^tm1(cre)Zjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Vip^tm1(cre)Zjh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Vip (MGI:98933)

• Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the vasoactive intestinal polypeptide locus (Vip). This construct was electroporated into C57BL/6 x 129S4/SvJae hybrid embryonic stem (ES) cells. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vip (mouse) Summary of all recombinase alleles driven by Vip. Comparative matrix view of recombinase activities Alliance mouse Vip gene page

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Vip Mutation: 24 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 251 reference(s)