Rnaseh2b^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnaseh2b^{tm1a(EUCOMM)Wtsi}
• Name: ribonuclease H2, subunit B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432164
• Synonyms: Rnaseh2b^KOF, Rnaseh2b^tm1a, RNaseH2^null
• Gene: Rnaseh2b Location: Chr14:62569517-62610445 bp, + strand Genetic Position: Chr14, 33.19 cM, cytoband C3
• Alliance: Rnaseh2b^{tm1a(EUCOMM)Wtsi} page
• IMPC: Rnaseh2b gene page

Mutation origin

• Mutant Cell Lines: EPD0087_4_A01, EPD0087_4_A02, EPD0087_4_A03, EPD0087_4_A04, EPD0087_4_B01, EPD0087_4_C01, EPD0087_4_C02, EPD0087_4_C04, EPD0087_4_D03, EPD0087_4_D04, EPD0087_4_E03, EPD0087_4_F03, EPD0087_4_G01, EPD0087_4_G03, EPD0087_4_H01, EPD0087_4_H02, EPD0087_4_H03, EPD0087_4_H04
• Germline Transmission: Earliest citation of germline transmission: J:186180
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 62590506 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 62591370. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Mice Carrying this Mutation:	7 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Rnaseh2b Mutation: 43 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 7 reference(s)