Dmxl2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dmxl2^{tm1a(EUCOMM)Wtsi}
• Name: Dmx-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432280
• Synonyms: Dmxl2^tm1a
• Gene: Dmxl2 Location: Chr9:54272442-54408910 bp, - strand Genetic Position: Chr9, 29.58 cM, cytoband C
• Alliance: Dmxl2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Dmxl2 gene page

Mutation origin

• Mutant Cell Lines: EPD0431_3_A05, EPD0431_3_A06, EPD0431_3_B07, EPD0431_3_B08, EPD0431_3_C06, EPD0431_3_C08, EPD0431_3_D06, EPD0431_3_D08, EPD0431_3_E07, EPD0431_3_E08, EPD0431_3_F05, EPD0431_3_F06, EPD0431_3_F08, EPD0431_3_G05, EPD0431_3_G06, EPD0431_3_H06
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 54367995 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 54368954. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Generation of the Dmxl2^tm1a(EUCOMM)Wtsi and Dmxl2^tm1c(EUCOMM)Wtsi alleles

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Dmxl2 Mutation: 122 strains or lines available

Notes

Cell line EPD0431_3_E07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:217623

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 10 reference(s)