About   Help   FAQ
Dmxl2tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Dmxl2tm1a(EUCOMM)Wtsi
Name: Dmx-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432280
Synonyms: Dmxl2tm1a
Gene: Dmxl2  Location: Chr9:54272442-54408910 bp, - strand  Genetic Position: Chr9, 29.58 cM, cytoband C
Alliance: Dmxl2tm1a(EUCOMM)Wtsi page
IMPC: Dmxl2 gene page
Mutation
origin
Mutant Cell Lines:  EPD0431_3_A05, EPD0431_3_A06, EPD0431_3_B07, EPD0431_3_B08, EPD0431_3_C06, EPD0431_3_C08, EPD0431_3_D06, EPD0431_3_D08, EPD0431_3_E07, EPD0431_3_E08, EPD0431_3_F05, EPD0431_3_F06, EPD0431_3_F08, EPD0431_3_G05, EPD0431_3_G06, EPD0431_3_H06
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 54367995 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 54368954. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Generation of the Dmxl2tm1a(EUCOMM)Wtsi and Dmxl2tm1c(EUCOMM)Wtsi alleles
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 3 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Dmxl2 Mutation:  122 strains or lines available
Notes
Cell line EPD0431_3_E07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:217623
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/10/2024
MGI 6.24
The Jackson Laboratory